Week 5 (9/26 – 9/30)
Finish cloning your gene into pNIC-Bsa4
For Virtual Refresher:
Since the blades are full - you only need to have your first Run started by the end of this week. (by Friday 9/30)
Begin realVirtual Screening - prepare protein in GOLD and start jobs (cb306 should be done this week and the next one started)
Wikispaces check on each Friday Week 6 (10/3 – 10/7)
Transform positive clone with your gene into BL21(De3) cells - begin expression
Continue realVirtual Screening - prepare protein in GOLD and start jobs
Lab Notebook check this Friday (7:00 pm)
Wikispaces check on each Friday
10/03/11 - Still need PyMol Refresher from: Joey O, Larry H., J. C. S. Week 7 (10/10 – 10/14)
Journal Club 3
Research Report Due - Tues Oct. 11th - Guidelines in GDocs folder
Virtual Screening help session for setting up your protein (should have started your second library this week)
Monday 4 - 5:30 in WEL 2.306 Computer Lab.
Express and Purify and Characterize your protein PyMol Image contest Due Friday at Midnight
Wikispaces check on each Friday Week 8 (10/17 – 10/21) VIRTUAL SCREENING - should have 3 libraries completed, at the least
FPLC purify enzyme, store enzyme
Test your enzyme for activity
Wikispaces check on each Friday
Week 9 (10/24 – 10/28)
Journal Club 4 VIRTUAL SCREENING - finish by this week - do Top Ligands Procotol to get final compounds for ordering
FPLC purify enzyme, store enzyme
Test your enzyme for activity Fri ( 10/28) Lab Notebook check this Friday(7:00 pm)
or Sat night if working on Sat.
Wikispaces check on each Friday
Protein Expression Scheduling:
Here's the Sign Up Form on Google - new guys and returning Spring VDSers should fill this out by Monday night.
Week 10 (10/31 – 11/4) Tues Nov. 1st Thursday Nov 3rd at Midnight (Materials & Methods for Cloning due) FRI External Open House - Wednesday Nov. 2nd 1- 4 pm Top Ligands Procotol to get final compounds for ordering
FPLC purify enzyme, store enzyme
Test your enzyme for activity
Wikispaces check on each Friday Week 11 (11/7 – 11/11)
Journal Club 5
Inhibition Assays
Tues Nov. 8th at Midnight (Materials & Methods for Virtual Screeningdue) - see Tips & Hints page
Wikispaces check on each Friday Week 12 (11/14 – 11/18) Tues Nov. 15th at Midnight (Materials & Methods for Expression, Purification, and Characterization due)
Purify and Characterize your protein - NOTE: this is a long and arduous process. Don't put it off.
Inhibition Assays Fri ( 11/18) Lab Notebook check this Friday(7:00 pm)
or Sat night if working on Sat.
Wikispaces check on each Friday
Protein Protocols to be completed - also see instructional videos on GDocs
ProtocolLabEnzymeTest_Phosphatase_VDS_Fall11.xlsx
Then on to Inhibition Assays once compounds arrive Week 13 (11/21 – 11/25): Thanksgiving
Journal Club 6
Inhibition Assays - on 2 compounds
Week 14 (11/28 – 12/2) - Last Class week
Inhibition Assays - on 2 compounds
Intro to ICM?
Tues Nov. 29th - FINAL PRESENTATIONS of Enzyme Inhibition Graphs - everyone
make a one slide PowerPoint and upload to the GroupMeetingSlides/FInalSlides_StudentsFall11 folder on GDocs
save file with your name and date in the file name
Thurs (12/1) - Lab Cleanup (Required) - each students spends 1 hr cleaning in lab (counts as 1 hour of lab time) Fri (12/2) - Lab Cleanup (Required) - each students spends 1 hr cleaning in lab (counts as 1 hour of lab time)
Sat ( 12/3) Final Report due (5:00 pm ) Final Lab Notebook check (7:00 pm)
Final Wikispaces check
FROM FALL 11
3rd Week (9/12 – 9/16)
PyMol Refresher - Due Tuesday 9/12
Science Works assignment due Friday Sept 16th - see announcement page
Lab Notebook Check this Friday- leave your notebook in 'Drug Screening Notebooks' drawer in lab by 7:00 pm
Wikispaces check on each Friday
4th Week (9/19 – 9/23)
Virtual Screen Refresher - Due 9/20 (see Announcement page for Virtual Screen Help Session on Thursday 9/14)
Wikispaces check on each Friday
Week 5 (9/26 – 9/30)
Finish cloning your gene into pNIC-Bsa4
For Virtual Refresher:
Since the blades are full - you only need to have your first Run started by the end of this week. (by Friday 9/30)
Begin real Virtual Screening - prepare protein in GOLD and start jobs (cb306 should be done this week and the next one started)
Wikispaces check on each Friday
Week 6 (10/3 – 10/7)
Transform positive clone with your gene into BL21(De3) cells - begin expression
Continue real Virtual Screening - prepare protein in GOLD and start jobs
Lab Notebook check this Friday (7:00 pm)
Wikispaces check on each Friday
10/03/11 - Still need PyMol Refresher from: Joey O, Larry H., J. C. S.
Week 7 (10/10 – 10/14)
Journal Club 3
Research Report Due - Tues Oct. 11th - Guidelines in GDocs folder
Virtual Screening help session for setting up your protein (should have started your second library this week)
Express and Purify and Characterize your protein
PyMol Image contest Due Friday at Midnight
Wikispaces check on each Friday
Week 8 (10/17 – 10/21)
VIRTUAL SCREENING - should have 3 libraries completed, at the least
FPLC purify enzyme, store enzyme
Test your enzyme for activity
Wikispaces check on each Friday
Week 9 (10/24 – 10/28)
Journal Club 4
VIRTUAL SCREENING - finish by this week - do Top Ligands Procotol to get final compounds for ordering
FPLC purify enzyme, store enzyme
Test your enzyme for activity
Fri ( 10/28) Lab Notebook check this Friday(7:00 pm)
or Sat night if working on Sat.
Wikispaces check on each Friday
Protein Expression Scheduling:
Here's the Sign Up Form on Google - new guys and returning Spring VDSers should fill this out by Monday night.Week 10 (10/31 – 11/4)
Tues Nov. 1st Thursday Nov 3rd at Midnight (Materials & Methods for Cloning due)
FRI External Open House - Wednesday Nov. 2nd 1- 4 pm
Top Ligands Procotol to get final compounds for ordering
FPLC purify enzyme, store enzyme
Test your enzyme for activity
Wikispaces check on each Friday
Week 11 (11/7 – 11/11)
Journal Club 5
Inhibition Assays
Tues Nov. 8th at Midnight (Materials & Methods for Virtual Screeningdue) - see Tips & Hints page
Wikispaces check on each Friday
Week 12 (11/14 – 11/18)
Tues Nov. 15th at Midnight (Materials & Methods for Expression, Purification, and Characterization due)
Purify and Characterize your protein - NOTE: this is a long and arduous process. Don't put it off.
Inhibition Assays
Fri ( 11/18) Lab Notebook check this Friday(7:00 pm)
or Sat night if working on Sat.
Wikispaces check on each Friday
Protein Protocols to be completed - also see instructional videos on GDocs
ProtocolProteinExpression_VDS_Fall11_ver2.docProtocolProteinPurificationVDS_Fall11.doc
ProtocolProteinCharacterizationVDS_Fall11.doc
ProtocolFPLC_GelFiltration_VDS_Fall11V_v2.docx
ProtocolLabEnzymeTest_Phosphatase_VDS_Fall11.xlsx
Then on to Inhibition Assays once compounds arrive
Week 13 (11/21 – 11/25): Thanksgiving
Journal Club 6
Inhibition Assays - on 2 compounds
Week 14 (11/28 – 12/2) - Last Class week
Inhibition Assays - on 2 compounds
Intro to ICM?
Tues Nov. 29th - FINAL PRESENTATIONS of Enzyme Inhibition Graphs - everyone
make a one slide PowerPoint and upload to the GroupMeetingSlides/FInalSlides_StudentsFall11 folder on GDocs
save file with your name and date in the file name
Thurs (12/1) - Lab Cleanup (Required) - each students spends 1 hr cleaning in lab (counts as 1 hour of lab time)
Fri (12/2) - Lab Cleanup (Required) - each students spends 1 hr cleaning in lab (counts as 1 hour of lab time)
Sat ( 12/3)
Final Report due (5:00 pm )
Final Lab Notebook check (7:00 pm)
Final Wikispaces check